Gene regulation in eukaryotes

Fundamentally Different Logic of Gene Regulation in Eukaryotes and Prokaryotes

the promoter sequences (which is related, but not equivalent to, DNA binding affinity). In vivo, an isolated promoter region is sufficient to initiate transcription at a rate comparable to that achieved in vitro. Moreover, the strongest promoters initiate transcription at the maximal rate, which is ultimately limited by the rate at which RNA polymerase elongates and clears the promoter, thereby...

TRANSFAC® and its module TRANSCompel®: transcriptional gene regulation in eukaryotes

The TRANSFAC database on transcription factors, their binding sites, nucleotide distribution matrices and regulated genes as well as the complementing database TRANSCompel on composite elements have been further enhanced on various levels. A new web interface with different search options and integrated versions of Match and Patch provides increased functionality for TRANSFAC. The list of datab...

Conservation of gene co-regulation in prokaryotes and eukaryotes.

Genes that are part of the same operon in prokaryotes, or have the same expression pattern in eukaryotes, are transcriptionally co-regulated. If genes are consistently co-regulated across distantly related organisms, the genes have closely associated functions. It has been shown previously that such genes have a strong tendency to belong to the same protein complex in prokaryotes, and we show b...

Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes.

The Tet repressor protein (TetR) regulates transcription of a family of tetracycline (tc) resistance determinants in Gram-negative bacteria. The resistance protein TetA, a membrane-spanning H+-[tc.M]+ antiporter, must be sensitively regulated because its expression is harmful in the absence of tc, yet it has to be expressed before the drugs' concentration reaches cytoplasmic levels inhibitory f...

Advanced Gene Mapping in Eukaryotes